![]() ![]() Finally, we identify underappreciated steps in snoRNA 3′end processing performed by RBM7. Moreover, we demonstrate that the two transesterification steps of pre-mRNA splicing temporally separate ALYREF and RBM7 binding to splicing intermediates, and that exon-exon junction density drives RNA 5′end binding of ALYREF. Regardless of function, all tested factors interact with nascent RNA as it exits RNAPII. We apply tiCLIP to the RNA export adaptor, ALYREF a component of the Nuclear Exosome Targeting (NEXT) complex, RBM7 and the nuclear cap binding complex (CBC). To remedy this, we here conduct temporal-iCLIP (tiCLIP), combining RNAPII transcriptional synchronisation with UV cross-linking of RNA-protein complexes at serial timepoints. Yet, our current understanding of this process in vivo primarily stems from steady state analysis. Dynamic RNA-protein interactions govern the co-transcriptional packaging of RNA polymerase II (RNAPII)-derived transcripts. ![]()
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